Assisted Conception
- Gamete and Embryo Donation
- Surrogacy
  Sperm bank
Invitro fertilization (IVF) is a technique in which egg cells are fertilized by sperm outside the woman's womb. IVF is a major treatment in infertility when other methods of achieving conception have failed . The process involves hormonally controlling the ovulatory process, removing ova (eggs) from the woman's ovaries and letting sperm fertilize them in a fluid medium. The fertilized egg (zygote) is then transferred to the patients uterus with the intent to establish a successful pregnancy.

The term in vitro, from Latin, is used, because early biological experiments involving cultivation of tissues outside the living organism from which they came, were carried out in glass containers such as beakers, test tubes, or petri dishes. Today, the term in vitro is used to refer to any biological procedure that is performed outside the organism it would normally be occurring in, to distinguish it from an in vivo procedure, where the tissue remains inside the living organism within which it is normally found. A colloquial term for babies conceived as the result of IVF is test tube babies.

Initially IVF was developed to overcome infertility due to problems of the fallopian tube, but it turned out that it was successful in many other infertility situations as well. The introduction of intracytoplasmic sperm injection (ICSI) addresses the problem of male infertility to a large extent

Assisted reproductive technology (A.R.T.) which includes (IVF, ICSI, ZIFT etc) now provides hope for many infertile couples who previously could not be treated. Basically the aim of ART treatments is to stimulate the ovaries to produce several follicles so that more than one egg can be retrieved during egg pick-up, fertilize them in the laboratory under strict and monitored conditions and put the fertilized egg back into the uterus, thus hoping to achieve a successful pregnancy in conditions where a natural pregnancy would be difficult or impossible to achieve.
Definitions Of Different Procedures
STANDARD I.V.F. (or In Vitro Fertilization)

The eggs are collected and placed in special culture medium. Prepared sperm is added (i.e. insemination) and then fertilization is assessed. The resultant embryos are transferred into the woman's uterus approximately 2-3 days after the egg pick-up.

This is the most common procedure.


The embryos are replaced into the uterus 2-3 days after egg pick-up (for standard I.V.F.)

A maximum of 3 embroys are replaced, to reduce the chance of multiple pregnancy, although sometimes there will be exceptions based on clinical grounds.


In this treatment one sperm is injected into the cytoplasm of an egg. It provides hope for couples who have had poor or absent fertilization in previous I.V.F. attempts and for men with severe male factor infertility.

ART treatments include procedures such as ZIFT (Zygote intrafallopian tube transfer), SIFT (Semen Intrafallopian tube transfer ) and a few others . However these are hardly ever done now with IVF and ICSI being the treatment in nearly all cases. Further advanced procedures which could be done along with IVF or ICSI include Assisted Hatching, Blastocyst Transfer, and Preimplantation Genetic Diagnosis

The TREATMENT CYCLE used during the IVF and ICSI procedures basically involves 5 steps and these will be discussed in detail.
Steps involved during the IVF and ICSI procedures
1. STIMULATION AND GROWTH OF OVARIAN FOLLICLES that contain the eggs or oocytes




1. Stimulation and growth of ovarian follicles
During a normal cycle, only one follicle is produced by your ovaries. The follicle is like a small bubble on the ovary which grows to 20mm diameter and contains one oocyte (egg) which is about O.lmm in diameter. When ovulation occurs, the follicle bursts and the oocyte is released into the end of the fallopian tube.

In a treatment cycle, the ovaries are stimulated by certain drugs to produce several follicles. This is done so that we have more number of eggs available for fertilization and can transfer more than one fertilized egg to increase the chances of conception

N.B. :- No medication other than that prescribed by your consultant should be taken whilst having treatment.


Stimulation and growth of ovarian follicles is usually achieved using gonadotropins which include Follicle Stimulating Hormone/FSH and hMG . These are used in conjunction with drugs like GnRh agonists or GnRH antagonists for proper regulation of the cycle. These drugs can be used in various combinations and dosages depending on what suits the patient.

There are three basic regimes for ovarian stimulation for IVF treatment. There is the 1."Flare" regime in which GnRH agonist is commenced on Day 2 of the cycle and FSH on Day 3 to initiate egg growth.

In the "Down Regulation" regime, the GnRH agonist is commenced at least 10 -14 days before the FSH.
Basically the Flare regime is a simpler and cheaper procedure for patients and it is very useful for women whose ovaries are slower in responding to FSH.
The Down Regulation cycle takes longer , but there can be smoother stimulation of the oocytes (eggs) with more oocytes maturing at the same rate and time.
3. In the "Antagonist" regime the gonadotropins are started on the second day of the cycle and the antagonist drug is started on the 6th day or when the follicles are around 12-13mm in size.

All this is done to produce more eggs at the same time making sure that there is no Lh surge (the release of hormone from the pituitary gland in the brain which causes the egg to get released or to become postmature). This is because when in a natural cycle only one egg matures, the cycle is well co-ordinated to make the egg release exactly at the time when it is mature. When multiple eggs are formed this co-ordination may get disturbed and hence to achieve control on the cycle a GnRH agonist or GnRH antagonist is used. Gonadotropins are the drugs which actually stimulate the ovaries to produce multiple eggs.

The dosage of FSH or hMG will be determined by your consultant and can vary between individuals and between cycles.

A TRANSVAGINAL ULTRASOUND SCAN is normally performed on Day 2 and then these scans are done regularly to assess follicular development. When the probe is placed in the vagina, an image of the ovaries can be seen on the scanning screen and fluid filled follicles that contain the eggs can be observed. The size of the follicle indicates the degree of egg maturity. When the egg is immature, the follicle is only a few millimeters in size, but when it is fully mature it grows to about 2 cms in size. When follicles are large in size, you may experience some discomfort in the region of the ovaries. Usually 5-15 follicles will develop but this varies considerably.

What is the process involved for the transvaginal scans
On the second day of the menstrual cycle or as determined by your doctor you will have to come for your first scan to determine that the ovaries are containing small follicles and that the uterine lining is as expected. If you are on the Long Protocol you may also have some scans off and on from the 21st day of your last menstrual cycle till you get your periods. Usually on the second day the Gonadotropins, that is the drugs which stimulate the ovaries are started. The woman will have to come for a scan at regular intervals as advised by the consultant. You will be given an appointment for your scan . You should pass urine and have an empty bladder prior to the scan. These are all OPD procedures and there is no need for admission during this.

During this period only the woman will have to come to IVF Pune as per the appointment and the husband need not be present everytime.

The woman will continue to take the injections as per the protocol during this period.When your consultant determines that a number of the follicles are mature, usually on Day 12 or 13 (although this may vary with Down Regulation), you will be given another injection of Human Chorionic Gonadotrophin/hCG which triggers ovulation approximately 36 hours later and therefore the timing of the egg retrieval is very exact.
Egg retrieval or egg pick-up
Egg (oocyte) Retrieval involves aspiration of the ovarian follicles under anesthesia in the operating theatre adjacent to the I.V.F. Laboratory at the IVFPune unit This is done by the transvaginal ultrasound technique. The anaesthetic for the ultrasound procedure is very light. For this procedure a probe is inserted into the vagina and this projects a picture onto a monitor which enables the doctor to direct a needle into each follicle to retrieve the eggs. The fluid filled follicles are aspirated under slight suction into a test tube which is passed on the the embryologist. The scientist or embryologist locates the eggs, places them into culture media and then into the incubator.
Preparation for the egg pick-up-informationregarding what is involved for you

All the instructions prior to the oocyte retrieval or egg pick-up will be given on the day the woman receives her hCG injection. The hCG injection is usually given late evening or in the night so as to schedule the pick-up in the morning around 34-36 hours later. On the day after the woman receives the hCG injection no stimulation drugs are to be taken. This is the day before the egg pick-up. Do not do any strenuous activity and abstain from sex. In fact the doctor will advise you regarding abstinence before the hCG is given. On the night before the egg pick-up the woman can have her dinner, but should not eat or drink anything after 10pm. She will have to come to IVF Pune the next day morning on an empty stomach, that is fasting. The time of reporting will be told to you along with all the other instructions. The husband will also have to come along with the wife as he will have to give the semen sample. Unless it is an ICSI procedure which also involves surgical retrieval of sperms the husband need not be fasting.
A written informed consent will have to be signed by the couple. As mentioned above the procedure is done in the IVF theatre under light anaesthesia.

After the procedure the woman will have to rest in the hospital for a few hours and will then be allowed to go home after ascertaining that all is fine. You will also be allowed to drink something after the effect of anesthesia wears out and can have a light meal at night. You will be started on progesterone supplementation from the day of the pick-up. see luteal phase support). You will also be told when to contact the unit and when to come back for the embryo transfer procedure.
As obvious this is a day procedure and there is no requirement for admission under normal circumstances.

Collection and preparation of semen sample. Insemination, fertilization of eggs and embryo culture
SEMEN SAMPLE :- Patients will be advised when the husband is required to produce a semen sample at the laboratory. Samples are assessed initially before processing, with the count, motility, quality of motility and proportion of morphologically normal sperm being recorded.
The sample is then processed by centrifuging or spinning the specimen in a test tube through a sterile solution which acts as a sieve to catch dead and abnormal sperm. Forward swimming sperm are able to pass through the interface and are collected from the bottom of the tube. These sperm are washed and used for IVF. Most patients are requested to have a "trial run" sperm preparation prior to their cycle to determine sperm quality and thus the best form of treatment.
INSEMINATION: - Oocytes are inseminated approximately 4 hours after egg pick-up using the microdrop technique. This involves suspending 100,000 sperm and one egg each in a tiny drop of culture media under sterile paraffin oil. This procedure confines the egg and sperm to a small volume, allowing more egg-sperm interaction. Thus the sperm and egg are fertilized outside the body, in the laboratory and cultured for 2-3 days.
FERTILIZATION:- The eggs are checked for fertilization 18-20 hours after insemination has been performed. Those eggs which either have not fertilized or which are abnormally fertilized (polyspermy) are discarded. Normally fertilized eggs (called pronuclear-stage embryos) are cultured for a further 24-48 hours for subsequent embryo transfer.
Embryos are assessed by looking at the stage of development (number of cells), the equality among the divided cells and the amount of fragmentation within the embryo. The embryos are graded. 2-3 embryos of a good grade are then used for transfer into the uterine cavity. Excess embryos can be frozen.

EMBRYO TRANSFER:- During embryo transfer, the woman's legs are rested on stirrups, a speculum is inserted in the vagina and the embryos are transferred into the uterine cavity by a very fine catheter which is passed through the cervical canal. Usually no anaesthetic is required. This is usually done approximately 2-3 days after Egg Pick-up and the procedure takes 5-10 minutes. We usually recommend bed rest for 2-6 hours, however, you will be allowed to get up to go to the toilet and have refreshments during this time.
You should have 48 hours rest at home after the embryo transfer and then you may return to your everyday activities. However, it is suggested that any strenuous exercise be avoided, and that sexual intercourse not be resumed until 1 week after embryo transfer. For those wishing to return to work, you may do so 2 days after the embryo transfer.
Notes for husbands on semen collection during the programme.
It is important to remember that sperm is constantly being produced and it takes 3 months to mature before it is ejaculated. You should keep this in mind 3 months prior to starting the programme. Smoking, alcohol, restrictive clothing and overheating such as saunas may all lower your sperm count. A general state of good health should be maintained. You may have intercourse during the treatment cycle, but please abstain from ejaculation for 2 days before your wife enters the hospital for her egg collection. Tests have shown that this period of abstinence produces the best possible sample. You should empty your bladder and wash your hands prior to producing the sample into the sterile jar provided. The sample should be produced at the IVF Pune unit for those undergoing the IVF procedure. In case you find it difficult to produce a sample at the unit, only then you may collect it at home and bring it to the laboratory provided it reaches the laboratory within half an hour of collection, earlier if possible.
Procedure side efficts

You may have a vaginal discharge after an ultrasound pick-up and if this persists or becomes offensive, please contact the IVF Pune unit.
Sometimes complications can arise from these procedures, relating either to the actual surgical procedure or to the anaesthetic and these can be further discussed with your consultant.
The use of ovarian stimulation drugs and since more than one embryo is transferred the chances of multiple pregnancy are present. All this will be discussed with the couple during the counseling session.

LUTEAL PHASE SUPPORT:- This is the treatment after the embryo transfer. However the progesterone supplementation is started in a low dose on the day of the egg pick-up.
As during the IVF treatment the ovaries are manipulated there is a possibility of inadequate hormonal support to help implantation and to the established pregnancy.
This may decrease the chance of a successful pregnancy or lead to an abortion of an established pregnancy if additional support with hormones is not given to the woman.
In order to avoid this you are given hormone drug supplements in the form of injections or vaginal suppositories which will provide adequate support to nurture the pregnancy. These should be taken daily as advised and not stopped without consulting your doctor.
We require you to do a pregnancy test 14 days after the embryo transfer procedure using the kit the unit has provided or the test can be done at the unit itself. The date when this test has to be done will be intimated before you leave the hospital after the embryo transfer procedure.

If the pregnancy test is positive, you will need to have an ultrasound scan at an appropriate time after the test so again, please contact us to organize this.

You may make an appointment to see your consultant at any time to discuss any queries you may have or to discuss future plans. Indeed we recommend that you do so if your treatment cycle is unsuccessful and a follow-up appointment will be arranged.
It is possible to start another IVF cycle after two further periods have elapsed or a frozen embryo cycle after one period.It is important that you contact the IVF Pune unit to notify us of the outcome of your treatment cycle.
The treatment cycle for the couple will remain as mentioned above. However certain micromanipulation techniques will be done or the embryo will be cultured longer in the following procedures.
  This is a technique developed in Belgium in the early 90's whereby one sperm is injected into the cytoplasm of an egg. The ability of ICSI to achieve higher fertilization and pregnancy rates regardless of sperm caharcteristics makes it a very important micromanipulation technique.
  Some of the indications when ICSI is done are
Very low sperm counts and motility
Surgical sperm retrieval in cases of azoospermia
Previous fertilization failure in IVF cycles
Retrieval of very low number of eggs

Cases where Preimplantation genetic diagnosis is to be done.

The technique of ICSI is very fine where the egg has to be held with a holding pipette and one single sperm is selected and injected inside with an injecting pipette. However, it should be considered that sub fertile men have a higher frequency of chromosomal abnormalities and the slightly increased risk of congenital abnormalities could also be related to the underlying cause on the male infertility.
ICSI pregnancy rates also depend on the quality of oocytes available from the woman and increasing maternal age and impaired oocyte quality can decrease ICSI-related pregnancy rates
Sometimes the scientists have to use the ICSI technique on patients who were previously considered appropriate for ordinary micro drop insemination. This can be due to factors such as poor quality semen or oocytes on the day of oocyte retrieval. Therefore all patients undergoing A.R.T. treatment are requested to sign a consent form for ICSI if they are agreeable to the procedure, even though the technique might not be performed.

Approximately five days after fertilization, the human embryo reaches a development stage termed the BLASTOCYST stage.
The embryos have to be cultured in the laboratory for a longer period of 5 days for the embryos to reach this stage. This allows the good quality embryos to reach the is stage and helps in embryo selection. Presently studies do not support the routine use of Blastocyst transfer in all patients. Patients should be aware that when the embryos are cultured for an extended period of time,the embryos may not survive this and may get arrested and there is a possibility that some patients by Day 5 will have no embryos for transfer.


The outer shell of the embryo (called the Zona Pellucida) can sometimes become hardened and thickened, resulting in the embryo having difficulty in being released to attach to the uterus.
It is possible to make an opening in the zona pellucida to help the embryo escape from its shell. This is called Assisted hatching. It is done in one of the following ways, by either cutting a slit in the zona using a fine glass needle or by drilling a hole in the zona using acidic culture medium or by using laser assisted hatching technique. It is hoped that this technique will improve the chance of embryos implanting, thus resulting in pregnancy. Women who are older than 37, with repeated adverse TVF outcomes, and/or have a thickened zona pellucida, may be assisted with this procedure. However, the technique may well result in a slightly increased chance of a multiple pregnancy because the embryo could split into two as it passes through the opening made in the zona. The implantation of more than one embryo increases the risk of problems occurring during the pregnancy. Presently Assisted hatching is indicated only in certain cases as mentioned above and whether it improves the live birth rate is under debate.